onesteptramadol

 

Our one Step Tramadol Urine Test is a great one step test for the qualitative detection of tramadol and its principal metabolites in human urine at specified cut-off level.

Intended Use

The One Step Tramadol Urine Test is a lateral flow chromatographic immunoassay for the detection of Tramadol in human urine at the cut-off concentration of 1000 ng/ml. This assay provides only a qualitative, preliminary analytical test result. A more specific alternative chemical method must be used in order to obtain a confirmed analytical result. Gas chromatography/mass spectrometry (GC/VS) is the preferred confirmatory method. Clinical consideration and professional judgement should be applied to any drug of abuse test result particularly when preliminary positive results are used.

Summary

Tramadol is used similarly to codeine, to treat moderate to moderately severe pain. It is a synthetic analog of the phenanthrene alkaloid codeine and, as such, is an opioid and also a prodrug. Tramadol and its metabolites are excreted primarily in the urine with observed plasma half-lives of 6.3 and 7.4 hours for tramadol and O-desmethyltramadol, respectively. Approximately 30% of the dose is excreted in the urine as unchanged drug, whereas 60% of the dose is excreted as metabolites.

Principle

One Step Tramadol Urine Test is a competitive immunoassay that is used to screen for the presence of tramadol and ites metabolites in a sample competivitly combied to a limited number of antibody-dye conjugate binding sites.

When the absorbent end of the test device is immersed into the urine sample, the conjugate, and flows across the pre-coated membrane. When sample drug levels are zero or below the target cut off (the detection sensitivity of the test), antibody-dye conjugate preventing the antibody-dye conjugate from binding to the drug-protein conjugate immobilized in the Test Region (T) of the device. This prevents the development of a distinct colored band in the Test Region (T), indicating a potentially positive result. To serve as a procedure control, a colored line will appear at the Control Region (C), if the test has been performed properly.